Objective The aim of the study was to observe the hemostatic and procoagulant effect of the Dencichine Injection on different experimental animal models. Methods Dencichine Injection was administered intravenously to 3 groups of animals of each species respectively with certain experimental injuries at dose levels of low， medium and high. Results The bleeding time and content of acute injured liver in rabbits were obviously reduced by Dencichine Injection treatment at a dosage of 1.00 mg/kg， as well as the bleeding time of mesenteric arteries cut in rats at 2.00 mg/kg. Also， the increased vascular permeability of capillaries in mice were decreased by administration of Dencichine Injection 2.5 mg/kg. In addition， the mice stomach injury index were significantly reduced in all 3 dosage of Dencichine Injection treated groups. Furthermore， with the treatment of 1 mg/kg Dencichine in rabbits， the prothrombin time（PT） was tend to be shortening， and the decrease rate of plasminogen activator inhibitor-1（PAI-1） were obviously slow down. Conclusion Intravenous administration with Dencichine Injection in multiple models on different animal species with experimental injury caused bleeding could be reduced significantly via promoting coagulation and inhibiting fibrinolysis.