Objective To study the mechanism of Shenmai injection enhancing the efficacy of oxaliplatin combined with anti-colorectal cancer by observing the effect of Shenmai injection combined with oxaliplatin on the expression of hypoxia inducible factor-1（HIF-1α） in colon cancer colon cancer xenografts in nude mice. Methods The method of transplanting tumor of LoVo cells in nude mice was established by literature method. Set up blank control group（n=8）， oxaliplatin group（8 mg/kg， n=8）， Shenmai injection group （10 mg/g， n=8） and combination therapy group（Oxaliplatin 8 mg/ Kg；Shenmai Injection 10 mg/g， n=8）. intraperitoneal injection and recording the tumor volume every other day. Plotting the tumor growth curve. Observing the effect of each drug group on the volume and weight of transplanted tumor in nude mice after 14 days of administration. tumor tissue was taken for tumor inhibition rate， and immunohistochemical method was used to detect the expression of hypoxia-inducible factor-1α（HIF-1α） and microvessel density （MVD） of CD31-positive tumor tissue. Results The inhibitory rate of combined chemotherapy of Shenmai injection and oxaliplatin was 46.9%. Combination therapy group can significantly reduce the expression of HIF-1α and reduce the microvessel density of tumor tissue. Conclusion Shenmai injection can enhance the effect of oxaliplatin against colonic malignant tumors， and its mechanism may be related to the inhibition of HIF-1α-mediated angiogenesis signal pathway.