Objective To investigate the effect of Danggui Buxue Decoction on NF-kB mediated inflammatory reaction in acute myocardial infarction model in rats with bone marrow stem cell transplantation. Methods Seventy-two healthy male Sprague-Dawley（SD） rats were selected for the study. Successful AMI models were established. All the subjects randomly selected 24 cases as the control group， the other 24 cases as the observation group， and the remaining 24 cases as the model group. The control group was treated with conventional saline gavage. The control group was treated with bone marrow stem cell transplantation. The observation group was treated with Danggui Buxue Decoction by gavage on the basis of the control group. After 2 weeks， the cardiac function， myocardial NF-kB protein expression， myocardial apoptosis， and NF-kB mediated inflammatory reaction were observed in each group. Results The LVIDs and LVIDd levels in the observation group and the control group were lower than those in the model group respectively， and the LVEF levels were higher than those in the model group. The difference was statistically significant（P<0.05）. The LVIDs and LVIDd levels were lower in the observation group than in the control group， and the LVEF levels were higher in the observation group than in the control group（P<0.05）. The expression of NF-kB and myocardial apoptosis index were lower in the observation group and the control group than in the model group， and the difference was statistically significant（P<0.05）. The expression level of NF-kB and myocardial apoptosis index were lower in the observation group than in the control group， and the difference was statistically significant（P<0.05）. The levels of IL-6 and TNF-α in the observation and control groups were lower than those in the model group， and the difference was statistically significant（P<0.05）. The levels of IL-6 and TNF-α in the observation group were lower than those in the control group， and the difference was statistically significant（P<0.05）. Conclusion Danggui Buxue Decoction is effective in the treatment of bone marrow stem cell transplantation in rats with acute myocardial infarction. It can reduce NF-kB mediated inflammatory response， effectively reduce myocardial cell apoptosis， improve myocardial injury in rats， and promote myocardial function recovery.