Objective To explore the mechanisms of acupuncture at “Shenshu”（BL 23） and “Zusanli”（ST 36） on the wnt/β-catenin pathway in the treatment of postmenopausal osteoporosis（PMOP） in rats. Methods Fifty female SD rats were randomly divided into a blank group， a sham-model group， a model group， a acupoint group and a non-acupoint group. The treatment group and non-acupoint group were given acupuncture once per day， for 30miniter， 10 treatments made one session. There were 5 days at the interval between two sessions. Six sessions of treatment were required. Bone mineral density were measured using a Dual Energy X-Ray Bone Denstormeter. Activity of alkaline phosphatase（ALP） in serum was measured by chromatometry. The expression of Wnt1， glycogen synthase kinase-3β（GSK-3β）， β-catenin， Dsh and mRNA in lumbar vertebra were detected by RT-PCR. Results Bone mineral density in the experimental group were higher than those in the model group and non-acupoint group. And serum ALP were lower than those in the model group. The expression of Wnt1， GSK-3β， β-catenin， Dsh and mRNA were significantly difference（P<0.05）. Conclusion Acupuncture at “Shenshu”（BL 23） and “Zusanli”（ST 36） possesses marked preventive and therapeutic effect for PMOP. This action is probably associated with the regulation of the key molecules of Wnt/β-catenin signaling pathway.