Objective To study the analgesic， anti-inflammatory activity and safety of Extract from Ligularia dictyoneura（Franch.） Hand.-Mazz（ELD）. Methods The acute toxicity reaction and mortality in mice were evaluated by acute toxicity assay. Pain models caused by acetic acid，hot stimulation and formaldehyde were used to observe the analgesic activity of ELD. Mouse ear swelling model induced by xylene was used to measure the anti-inflammatory activity of ELD. Results The LD50 of ELD was 3.65 g/kg and the 95% confidence limit was 3.30 g/kg-4.04 g/kg. After the administration，animals showed some toxic reactions， such as： pronate， exercise reduction， movement disorders， diarrhea， tremor， breath with difficulty. Compared with the vehicle group， the ELD group could significantly inhibit the pain response of mice induced by acetic acid， hot stimulation and formaldehyde. Moreover， the ELD group could markedly inhibit mice ear swelling caused by xylene. Conclusion The Extract of Ligularia dictyoneura（Franch.） Hand.-Mazz showed a certain effect on inflammation and pain.