Objective To analyse on the components of eluted fractions of Panax notoginseng（Burk.） F. H. Chen. Screening the active fractions by observing the hemostatic effect， antithrombotic effect， activating blood circulation and resolving stasis effect. Methods The medicinal materials were extracted by 70% ethanol， and fractions prepared by macroporous resin. The content of total saponins was determined by UV spectrophotometry， the content of dencichine and five major saponins were determined by HPLC. Bleeding time was measured by tail-cutting method， coagulation time was measured by the slide method. Collagen-adrenalin was used to induce thrombus in mice. Rats with acute blood stasis model were established by intravenous injection of high molecular dextran. Results The samples were prepared， included： the extract， water eluted fraction， 30% and 70% ethanol eluted fraction. The content of notoginseng total saponins were 32.51%， 0%， 52.39%， 83.61%. The dencichine exists in water eluted fraction only（2.78%）. The extract and fractions could shorten the bleeding and coagulation time significantly. However， the water eluted could shorten coagulation time more significantly. The extract and 30% ethanol elution fraction could improve the recovery rates of hemiplegic mice significantly. Extract， 30% and 70% water eluted fractions could decrease the blood viscosities at all shear rates， significantly. Conclusion The dencichine only exists in water eluted fraction， saponins exist in 30% and 70% ethanol eluted fractions， but their contents and types were different. Water eluted fraction is main active fraction on hemostasis. The 30% ethanol eluted fraction show the obvious antithrombotic effect， and it’s main active fraction. The 30% and 70% ethanol eluted fractions show the obvious activating blood circulation and resolving stasis effect， and they are the main active fraction.