Objective Systematic evaluation of the clinical efficacy of acupuncture in the treatment of premature ovarian failure（POF）. Methods Computer search for China Knowledge Network（CNKI）， Wanfang database（WanFang）， Weipu database（WeiPu）， PubMed and other databases， published from the time of database construction until January 1， 2019， the language of the literature is Chinese and English， search for acupuncture. The literature for the treatment of POF was included in the study literature according to the inclusion criteria， the included literature was evaluated using the Jadad scale and Cochrane HandBook 5.1.0， and the meta-analysis was performed using RevMan 5.3. Results There were 10 research articles included in the study， a total of 690 patients with POF. Meta analysis results show：The cure rate was statistically significant（P=0.01）， the cure rate of the acupuncture group was significantly higher than that of the western medicine group. The effective rate was not statistically significant（P=0.53）， the acupuncture group was similar to the western medicine group. The significance of learning（P<0.00001）， the inefficiency of acupuncture group was significantly lower than that of western medicine group；follicle stimulating hormone（FSH） was statistically significant（P<0.0001）， acupuncture group could significantly reduce serum FSH value；luteinizing hormone（LH） and estradiol（E2） were not statistically significant（P=0.97， P=0.55）. The acupuncture group and the western medicine group reduced LH and increased E2 levels. Conclusion Acupuncture treatment can significantly improve ovarian function and serum sex hormone levels in patients with POF.