Objective With the frequent occurrence of the adverse reactions of injection， it is gradually noticed that the excipient polysorbate 80 for injection was the important cause. Based on analysis on literature reports， injection containing polysorbate 80 played an important role in anaphylactoid reactions. It can be also deduced that macromolecular impurities could be the main substances that induced safety problems. The article suggested that the safety can be improved if the macromolecular impurities in polysorbate 80 for injection were removed.