Objective To study and control quality of Aurantii Fructus in Yangyin Kouxiang Mixture． Methods Thin layer chromatography（TLC） was used for qualitative identification of Aurantii Fructus． High performance liquid chromatography （HPLC） was used for content determination of naringin and neohesperidin in Aurantii Fructus（AF） on a Waters Xbridge C18（250 mm × 4．6 mm，5 μm）， and the mobile phase was acetonitrile-water（20 : 80） at the column temperature of 30 ℃． The flow rate was 1.0 mL·min-1 and the detection wavelength was 283 nm． Results The TLC spots were clear and showed a good separation． HPLC showed the calibration curve of naringin had a good linear relationship in the mass range of 0.05680 μg～0.7100 μg（r=0.9999） and the average recovery was 100.21%; the calibration curve of neohesperidin had a good linear relationship in the mass range of 0.03053 μg～0.3817 μg（r=0.9999） and the average recovery was 98.33%． Conclusion The method is accurate with good reproducibility， which can be used for the quality control of Aurantii Fructus in Yangyin Kouxiang Mixture.