Objective To investigate the effect of 80% ethanol extract of GastrodiaeRhizoma (hereinafter referred to as “extractive from ethanol extract of GastrodiaeRhizoma”) on angiogenesis in ischemic hindlimbs of mice, and to provide experimental basis for the treatment of ischemic diseases of lower extremity. Methods 72 male mice were randomly divided into the model group, the sham operation group and the ethanol extraction group with different treat time. The model of lower limb ischemia was established by blocking femoral artery. After modeling the mice were continuously administrated with ethanol extract of Gastrodiae Rhizoma 0.94 g/kg. The blood flow of ischemic was measured with a laser Doppler blood flow (LDBF) analyzer on the 1, 3, 7 and 14 d after giving the samples. The expressions of vascular endothelial growth factor A (VEGF-A), vascular endothelial growth factor receptor 2 (VEGFR-2) and angiopoietin 2 (Angpt2) mRNA were detected by performing qPCR. The neovascularization of gastrocnemius muscle was labeled by immunofluorescence triple labeling (Ki67/Tomato-Lectin/DAPI) at the 7 and 14 d after operation. Results Compared with the model group, the blood flow and the positive expression of Ki67 in the ethanol extract of Gastrodiae Rhizoma group were increased on the 3 and 7 d after operation(P<0.001). The expressions of VEGF-A and Angpt2 mRNA on the ischemic side were up-regulated on the 1 and 3 d after operation. On the 7 d after operation, the expressions of VEGFR-2 and Angpt2 mRNA were up-regulated(P<0.05). Conclusion Ethanol extract of Gastrodiae Rhizoma may promote angiogenesis in mouse lower limb ischemia model, and its mechanism may be related to promoting the expression of angiogenic factor VEGF-A and its receptor 2 and Angpt2.