Objective To explore the possible related factors in the pathogenesis of acne mastitis. Methods According to the designed questionnaire, 270 patients with acne mastitis were investigated and analyzed by Microsoft Office Excel 2007. Results According to the incidence of acne mastitis in this study, the possible related factors are from high to low in the following five aspects: milk collaterals(mammary ducts) obstruction, breast injury, improper diet , menstrual cycle and information about breast-feed after childbirth, the other related factors are history of hyperprolactinemia, mental diseases, polycystic ovary syndrome, as well as stress, fatigue, etc. Conclusion The related factors of acne mastitis include two aspects: pathogenic and predisposing factors The pathogenic factor is the accumulation of secretion(acne like secretion) in the milk collaterals, hyperprolactinemia, and the most common predisposing factors may encompass milk collaterals obstruction, trauma, improper diet, ectinducements mainly include trauma, fatigue, and improper diet, etc.