Objective To predict the potential target of Danzhi Xiaoyao Powder（DZXYP） treatment of Polycystic Ovary Syndrome（PCOS） by the pharmacological method of network, so as to explore the pharmacokinetic mechanism of the DZXYD. Methods The main active components of DZXYD were screened by TCMSP database, and the relevant targets were predicted using PubChem database and SwissTarget Prediction. Gene Cards are used to mine known PCOS gene targets. Combined with the drug’s predicted target and disease target, the String database was constructed to construct the drug-disease target protein interaction network. Finally, the DAVID database and Cytoscape were used to analyze the biological functions and metabolic pathways of key nodes. Results A total of 154 active components were screened by DZXYP, and 483 related targets were predicted. After data retrieval, 3 619 PCOS-related targets were obtained, and there were 140 intersections with drugs. A total of 286 biological processes(BP) , 81 molecular functions(MF) , 34 cellular components(CC) and 83 KEGG pathways were enriched for key targets. Conclusion DZXYP may act on multiple targets, regulate multiple signaling pathways such as phosphatidylinositol 3-kinase/protein kinase B（PI3K/Akt）, ovarian steroid hormone synthesis, insulin resistance（IR）, improve the inflammatory state of PCOS, restored insulin Sensitivity and reducted the synthesis of androgen. Thereby playing a role in the treatment of PCOS. It provides a new interpretation of the mechanism of action of DZXYP and provides new ideas for further research on its pharmacology.