Objective To investigate the effects of Sijunzi Ointment Granule on myocardium, SOD, MDA in spleen qi deficiency rat model. Methods 60 SD rats were randomly divided into blank group, model group, Sijunzi decoction group, Sijunzi ointment low, medium and high dose groups, with 10 rats in each group. The method of compound factor modeling was adopted, which is, losing diet, exhausting gas and exhaustion working. In the blank group, there is no model making, normal diet and normal saline intervention, others were fed every other day. Model group and drug groups were given rhubarb solution in the morning and forced to swim until exhaustion in the afternoon. Then normal saline intervention in model group, Sijunzi Ointment were administered by gavage at the concentrations of 1.8, 3.6, 5.4 g/kg, and Sijunzi Decoction （2.95 g/kg）, respectively. On the 29 d, samples were collected, and the changes of myocardial tissue structure were observed, and SOD and MDA were determined in myocardial homogenate. Results In the blank group, the myocardial tissue fibers were arranged neatly and orderly, and the model group were disordered with heavy edema, while the myocardial structural damage were relieved in the drug administration groups. Compared with blank group, the SOD activity decreased and MDA increased significantly in model group（P<0.05）. After administration, SOD activity was increased and MDA was decreased in drug groups（P<0.05）. Both the Sijunzi Decoction and Ointment low dose group have the same effect, and Sijunzi Ointment at high dose has the best effect. Conclusion Sijunzi Ointment Granule could improve qi and strengthen spleen, alleviate the damage of myocardial structure, increase SOD activity and decrease MDA, and protect the myocardium. The curative effect of granulated ointment is worthy and granules can be used in the preparation of ointments.