Objective To reevaluate the methodological quality and evidence quality of systematic reviews(SRs) of acupuncture for chronic fatigue syndrome(CFS). Methods PubMed, EMBASE, the Cochrane Library, web of science, VIP, CNKI and Wanfang database were searched by computer to collect the SRs of acupuncture and moxibustion in the treatment of chronic fatigue syndrome. The retrieval time limit was from the establishment of the database to December 1, 2019.The AMSTAR 2 scale and GRADE tool were used to evaluate the methodological and evidence-based quality of the inclusion SRs. Results Finally, 13 SRs were included, including 54 main outcome indicators. The results of the AMSTAR 2 tool evaluation showed that: 8 were of “low” quality and 5 were of “very low” quality. The results of the GRADE system showed that among the main outcome indicators included, there were 8 “high level” evidence quality grades, 10 “medium level” evidence quality grades, 6 “low level” evidence quality grades and 30 “very low level” evidence quality grades. Conclusion The current systematic review of acupuncture and moxibustion for CFS is of low quality and evidence level. Therefore, in the design of clinical trials in the future, large samples and high-quality RCTs should be used to strictly evaluate the efficacy and safety of acupuncture in the treatment of CFS, and the methodological quality and evidence quality of evidence-based acupuncture research should be further standardized in order to guide clinical in terms of decision-making and better use of the advantages and characteristics of Chinese medicine.