Objective To observe the effect of Sanguisorba Plaster on wound healing in a rat model of deep II degree burn and explore its action mechanism. Methods SD Rats were randomly divided into normal group, model group, Mei-bao’s scald-plaster group, Mupirocin Ointment group and Sanguisorba Plaster group. All rats except the normal group were made deep II degree scald model, and the wound healing rate, the change of histopathology, the relative expression of CD31 protein and the levels of IL-6 and TNF-α in serum were detected. Results On 7, 14, 21 d after burn, the wound surface and histopathology of rats in Sanguisorba Plaster group were good, followed by Mei-bao’s scald-plaster group, Mupirocin Ointment group and model group. Compared with normal group, the levels of IL-6 and TNF-α in serum and CD31 in tissues were increased(P<0.05). And the wound healing rate and relative expressions of CD31 in drug treatment groups were significantly higher than those in model group(P<0.05), and the levels of IL-6 and TNF-α in serum were significantly lower than model group(P<0.05). On 14 d and 21 d after burn, the wound healing rate and relative expression of CD31 were significantly higher in Sanguisorba Plaster group than in Mupirocin Ointment group(P<0.05), while there was no difference when compared with Mei-baos scald-plaster group . Conclusion Sanguisorba Plaster could inhibit the release of inflammatory cytokines IL-6 and TNF-α, and enhance wound microangiogenesis, promote wound healing.