Based on UPLC-MS/MS detection platform, software Analyst 1.6.3 was used to process mass spectrum data, and combined database and multivariate statistical analysis was used to study the metabolic characteristics of Rosa L.‘Crimson Glory’（DH） and R.gallica L.‘Dianhong’（ZY）. A total of 174 flavonoids metabolites were detected, including 67 flavonoids(39%) and 37 flavonols(21%). Finally, KEGG data(Kanehisa et al. 2000) were used to analyze and find that differential metabolites were significantly enriched in the biosynthesis of flavonoids and flavonols, flavonoid biosynthesis metabolites.In the flavonoid biosynthesis pathway, the(-) -epigallocatechin, vindicin, kaempferol, luteolin, pine flavanone and apigenin were significantly up-regulated. Pentahydroxy flavone and (-)-Epiafzelechin was significantly down-regulated. In the biosynthetic pathway of flavonoids and flavonols, vindicin, eugenol, luteolin, Vindicin -2-o-rhamnoside, luteolin -7-o-glucoside, apigenin and golden sainfoin, Acacetin were significantly up-regulated. These different metabolites and corresponding metabolic pathways regulated the difference in the color of DH and ZY. Through metabonomics method discussed the differences of two edible roses in flavonoids metabolic group, reveal the reason of the different color of rose, for the further development of roses colors and the food we provide reference.