Objective To observe the effect of abdominal acupuncture combined with moxibustion on the levels of sex hormone and immune cells in patients with premature ovarian failure. Methods Totally 60 patients were selected and randomly divided into two groups, the western medicine group and the acupuncture group. The western medicine group was treated with keling Mongolian therapy, and the acupuncture group was given abdominal acupuncture combined with moxibustion. All patients were followed up for 6 months, and the clinical efficacy, as well as the levels of sex hormone and immune cells were observed at 3 and 6 months. Results The efficient of acupuncture treatment group is higher than western medicine group, but there was no statistically significant difference(P>0.05). The levels of estradiol(E2) and CD4+, CD4+ / CD8+ were increased obviously, while the progesterone follicle-stimulating hormone(FSH), luteinizing hormone (LH), and CD8+ levels were decreased after treatment, and it had significant difference as compared with the western medicine group(P<0.05). Moreover, the risk of adverse reactions had a low tendency as treated by abdominal acupuncture combined with moxibustion(P<0.05). Conclusion Abdominal acupuncture combined with moxibustion in the treatment of premature ovarian failure can alleviate the clinical condition of patients, improve the levels of sex hormones and immune system indicators and the clinical efficacy