Objective To explore the therapeutic effects of alcohol extract of Pu’er tea on ICR mice model with non-alcoholic fatty liver disease(NAFLD). Methods NAFLD model was successfully induced in ICR mice by high-fat diet. Different doses of alcohol extract of Pu’er tea（5.0、2.0 g/kg） were administrated to model mice for 6 weeks started from the last day of induction. Body weight of all mice were recorded during the experiment. At the end of the treatment, liver index, lipid levels both in the serum and liver tissue, and levels of both aspartate aminotransferase(AST) and alanine aminotransferase (ALT) were measured. Liver histological examination was performed to observe the morphological changes. Results Compared with the model group, significant decreases in body fat, liver index, blood lipid levels, levels of ALT and AST in the liver and serum were observed in the Pu’er tea treatment groups in a dose-dependent manner. Histopathological examinations of the liver sections in the high dose group revealed that hepatocellular steatosis was significantly improved compared with that in the model group, while the improvement was not as obviously as in the low dose group. Conclusion The alcohol extract of Pu’er tea significantly improved the hyperlipidemia and hepatocellular steatosis in NAFLD mice model induced by high-fat diet, thus showing great potential in the management of NAFLD.