Objective To explore the effect of an Yunnan national drug Qilongtian on the levels of Nrf2 and DJ-1 in chronic obstructive pulmonary disease (COPD). Methods 60 SD rats were randomly divided into normal control group, model group, positive control group, Qilongtian low, medium and high dose groups, with 10 rats in each group. The COPD model was induced by tracheal injection of lipopolysaccharide plus smoked cigarettes, and normal group was injected with saline. Qilongtian low, medium and high dose treatment groups were given 0.35, 0.7 and 1.4 g/kg Qilongtian by gavage respectively, and positive control group was given 13 mg/kg Montelukast Tablets. The number of nuclear cells in the alveolar lavage fluid were recorded, lung function indexes, levels of Nrf2 and DJ-1 in serum and lung tissue homogenate were measured. Results Compared with the normal group, the number of nuclear cells in the alveolar lavage fluid in the model group was significantly increased (P<0.05), lung function was impaired, meanwhile the levels of Nrf2 and DJ-1 in serum and lung tissue homogenate were decreased(P<0.05). After Qilongtian treatment, the lung function of rats were improved, and the levels of Nrf2 and DJ-1 were gradually increased in a dose dependent manner. Compared with the model group, the changes of medium and high dose groups showed statistical significance(P<0.05). Conclusion Qidongtian could optimize the redox homeostasis in the body by regulating the levels of Nrf2 and DJ-1, which leads to a therapeutic effect on COPD rats.