Objective To observe the effects of Daitongxiao ethanol extract (DTXEE) on hyperuricemia mice and gouty arthritis rats. Methods 60 mice were randomly divided into normal control group, model group, Benbromarone group and low, medium and high dose groups of DTXEE. The model group and drug groups were given 30 g/kg yeast by gavage, while the normal control mice were given distilled water. On the 4th day after modeling, the low, medium and high DTXEE groups were given 2.6, 7.8, 23.4 g/kg DTXEE, respectively, and the model group and normal control group were given distilled water. The Benbromarone group was given 0.02 g/kg benbromarone on the 6th day, administration lasted for 4 d. The blood was taken from the eyeball 1 h after the last administration. 60 Wistar rats were randomly divided into normal control group, model group, colchicine group, and low, medium and high dose groups of DTXEE. The DTXEE groups were gavaged with 1.8, 5.4, 16.2 g/kg of DTXEE respectively for 5 d before modeling, the colchicine group was gavaged with 0.28 mg/kg colchicine for 3 d before modeling, the normal control group and model group were given distilled water once a day. The acute gouty arthritis model was made 1 h later after the last administration in model and drug groups, 5 h after modeling, rats were anesthetized and blood was taken from abdominal aorta. The levels of UA, XOD, CRE, BUN, IL-6, TNF-α and IL-1β in the serum of rats and mice were detected by the kits, and the joint swelling of rats before and after modeling were observed. Results Compared with the normal control group, UA, XOD, CRE, BUN, IL-6, TNF-α, IL-1β in the model group were significantly higher (P<0.05). Restlessness, elevation of right foot, local redness and swelling, increased skin temperature and joint swelling index were observed in acute gouty arthritis rats. Compared with model group, the improvement of CRE in hyperuricemia and UA, CRE, IL-6, TNF-α in acute gouty arthritis were not significant after the intervention of low-dose DTXEE. While the levels of UA, XOD, CRE, BUN, IL-6, TNF-α, IL-1β levels were significantly lower in other drug groups(P<0.05). Moreover, the local joint swelling of rats with acute gouty arthritis in drug groups were improved better than model group. Conclusion DTXEE could reduce uric acid, improve renal function and anti-inflammatory in hyperuricemia mice and acute gouty arthritis rats.