Objective To observe the intervention effect of Shendi granule on miRNA-193a and WT-1mRNA in renal tissue of C-BSA rats. Methods Forty male SD rats were randomly divided into normal group (n=10), model group (n=10), Shendi granule group(n=10) and valsartan group(n=10). The rats in the Shendi granule group were given intragastric administration of Shendi granule aqueous solution 4.0 g/(kg·d), the valsartan group was given valsartan capsule suspension 20 mg/(kg·d), and the normal group and model group were given normal saline, once a day, once a day. After 8 weeks of continuous administration, the animals were treated, urine and kidney samples were collected. The concentrations of urinary albumin and creatinine, the contents of urinary Nephrin and Podocalyxin were detected by enzyme-linked immunosorbent assay (ELISA), and the expression of WT-1 and miRNA-193a mRNA in renal tissue was detected by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction(qRT-PCR). The ultrastructure of glomeruli was observed by transmission electron microscope, and the average thickness of glomerular basement membrane(GBM)(TGBM) was calculated. Results Compared with the normal group, the ratio of urinary albumin to creatinine(UACR), the contents of urinary Nephrin and Podocalyxin, the expression of miR-193amRNA and TGBM in kidney tissue were significantly increased, while the expression of WT-1mRNA was significantly decreased in the model group. Compared with the model group, the contents of UACR, urinary Nephrin and Podocalyxin, the expression of miR-193amRNA and TGBM in kidney tissue were significantly decreased, and the expression of WT-1mRNA was significantly increased in Shendi granule group and valsartan group, and the changes of UACR, urinary Podocalyxin, miR-193a and WT-1mRNA expression and TGBM in Shendi granule group were significantly better than those in valsartan group (P<0.05). The structure of glomerular podocytes in the normal group was normal. In the model group, most of the foot processes fused or disappeared, the basement membrane thickened diffusely, and large electronic dense deposits could be seen under the epithelium. The podocyte structure of Shendi granule group and valsartan group was better than that of model group. Conclusion Shendi granule may reduce podocyte injury and reduce urinary protein by inhibiting the expression of miR-193a and up-regulating the expression of WT-1 in renal tissue of C-BSA rats.