Objective To explore the effect of Weifuchun Capsule extract on cell apoptosis in human gastric cancer MGC-803 cells and the mechanism. Methods MGC-803 cells were treated with Weifuchun Capsule extract in different concentration. Relative cell viabilities were determined by the CCK-8 method; Hoechst 33258 staining was used to observe the morphological changes of cells; apoptosis and cell cycle were analyzed by flow cytometry; the protein expression levels of Bcl-2, Bax, Caspase-3, and Caspase-9 were detected by Western blot. Results Weifuchun Capsule extract (0～100 μg/mL) significantly inhibited the viabilities of MGC-803 cells in a dose- and time-dependent manner. Hoechst 33258 staining showed that the membrane permeability of Weifuchun Capsule extract treated MGC-803 cells increased. Flow cytometry results showed that Weifuchun Capsule extract could induce apoptosis in MGC-803 cells, the apoptotic ratios are (20.4 ± 2.7)% (25 μg/mL), (41.3 ± 6.2)% (50 μg/mL), (68.8 ± 5.8)% (100 μg/mL), significantly higher than that of control group (3.8 ± 1.6)% (0 μg/mL) (P<0.05). After different concentrations of Weifuchun Capsule extract treatment for 24 h, the proportion of MGC-803 cells in G0/G1 phase increased, while the proportion of the G2/M phase and S phase reduced. In 100 μg/mL Weifuchun Capsule extract treatment group, the proportion of cells in G0/G1 phase increased from 46.52% to 73.54%(P< 0.01). The data of Western blot showed that Weifuchun Capsule extract(25, 50, 100 μg/mL) up-regulated the expression levels of cleaved Caspase-3, cleaved Caspase-9, and Bax, but down-regulated Bcl-2, in a dose-dependent manner. Conclusion Weifuchun Capsule extract could inhibit the proliferation of MGC-803 cells and promote apoptosis, which may be associated with the down regulation of Bcl-2 expression and up regulation of Bax expression, as well as the increase of relative activity of Caspases.