Objective To investigate the effect and mechanism of the combination of Salvianolic Acid（SAL） and Panax Notoginseng Saponins（PNS） on the protection on astrocytes mitochondria and promotion of the expression of neurotrophic factor after Oxygen Glucose Deprivation/Reoxygenation（OGD/R） injury. Methods The activity of primary cultured rat astrocytes were detected by Cell counting Kit-8(CCK-8) method. The release of reactive oxygen species(ROS), intracellular Ca2+ concentration and mitochondrial membrane potential(MMP) were detected by flow cytometry. And real-time quantitative polymerase chain reaction(RT-PCR) and western blot（WB） methods were used to detect HIF-1α, PI3K/Akt/mTOR signaling pathway proteins, brain-derived neurotrophic factor (BDNF), nerve growth factor(NGF), insulin-like growth factors (IGF-1α). Results The OGD/R condition of glial cells was determined as OGD 4h/R24h and the concentration of SAL25 μg·mL-1 and PNS6.25 μg·mL-1 were determined. Compared with OGD/R group, the combined use of SAL25 μg·mL-1, PNS 6.25 μg·mL-1, SAL25 μg·mL-1 and PNS 6.25 μg·mL-1 could enhance the activity of astrocytes after injury, increase the MMP of astrocytes after injury.Decreased ROS release, decrease HIF-1α mRNA and protein expression in astrocytes after injury, increase Phosphatidylinositide 3-kinases(PI3K) phosphorylation level(P＜0.05). In addition, SAL also can increase the phosphorylation level of mTOR protein, and PNS increase the phosphorylation level of Akt, and significantly increased the expression of BDNF mRNA in injured astrocytes(P＜0.05).Combined use of SAL and PNS significantly increase the mRNA expression of BDNF, NGF and IGF-1α in astrocytes after injury(P＜0.05). Conclusion Combined salvianolic acid and pantoginseng saponins can protect the mitochondria of astrocytes after OGD/R injury, promote the expression of neurotrophic factors BDNF, NGF and IGF-1α.