Objective To explore the activation of mast cells in the different acupoints area of acute myocardial ischemia rats. Methods Twelve healthy male SD rats were stochastically assigned to control group and model group, with 6 rats per group. Acute myocardial ischemia was produced in rats by injecting 60 mg/kg of isoproterenol subcutaneously at multiple points. Myocardial ischemia proportion was measured by triphenyltetrazolium chloride(TTC) staining to verify the success of the model. Distribution and activation of mast cells in the left Neiguan (PC6) and in the left Xinshu (BL15) of rats was detected by toluidine blue staining. Results 1. The myocardial ischemia area of model group was significantly higher than it of control group（P<0.05）; 2. Compared with the control group, the number and degranulation rate of MCs in the left Xinshu of model group were significantly increased（P<0.05）; the degranulation rate of MCs in the left Neiguan of model group was significantly increased（P<0.05）, there was no significant difference in the number of MCs in the left Neiguan between 2 groups; 3. The number of MC in the left Neiguan was higher than that in the left Xinshu among control group （P< 0.05）, no significant difference in the number of MCs between that 2 acupoints among model group. Conclusion Xinshu(BL15) and Neiguan(PC6) in the acute myocardial ischemia model rats were sensitized, which displayed visible recruitment and degranulation of MCs; The number of MCs was different in acupoints of different body parts, that in limbs acupoints probably higher than that in back acupoints.