In order to observe the effect of food intake on serum uric acid(SUA), male uricase-deficient rats (Kunming-DY rats) aging 45 days were randomly divided into fasting group (fasting for 2 days) or dieting groups (90%, 80%, 75%, 70%, 60%, 50% and 25% of normal food intake for 14 days). Before, during and after the experiment, blood samples were drawn from the tail to prepare serum. In the same time, body weight, 24-hour food intake and water consumption were recorded, and 24-hour urine and feces of rats were collected. The uric acid in samples was detected by uric acid assay kits of phosphotungstic acid method. The results showed that SUA in fasting group significantly increase. SUA in the rats that received 75% of the normal food supply was lowered. Besides, creatinine, total cholesterol and triglyceride in their serum decreased significantly after 2-weeks' treatment, with their stable body weight. Other food intakes had almost no significant effect on, or increase SUA in Kunming-DY rats. In conclusion, 75% food intake lower SUA in uricase-deficient rats with their improvement of renal function and blood lipid.