Objective To observe the effects of electroacupuncture "Baihui (DU20)" and "Yintang (EX-HN3)" on the behavior and expression of mTOR and GSK-3β in prefrontal cortex of Wistar-Kyoto(WKY) rats. Methods Thirty male WKY rats were randomly and equally divided into an electroacupuncture group, sham-acupuncture group and model group. Another 10 male Wistar rats were set as a normal group. The normal and model groups were normally fed routinely for 21 days without any intervention, and the sham group was treated with sham-acupuncture for comfort. In the electroacupuncture group, the rats were treated at "Baihui (DU20)" and "Yintang (EX-HN3)", and the behavioral changes were evaluated by their body mass, open field test and sugar-water preference test after 21 days. The expression levels of mTOR and GSK-3β in the prefrontal cortex were measured by Western Blot. Results Compared with the normal group, the body mass, horizontal movement in the open field experiment, vertical movement and sucrose water intake ratio of the model group were significantly lower than those of the normal group; compared with the sham group, the body mass, horizontal and vertical movement in the open field and sucrose water intake ratio of the rats in the electroacupuncture group were significantly increased; the expression of mTOR in the prefrontal cortex was higher in the electroacupuncture group than that in the sham group, and expression of mTOR in the normal group was higher than that in the model group. The expression of GSK-3β in the prefrontal cortex in the electroacupuncture group was lower than that in the sham group, and the expression of GSK-3β in the normal group was lower than that in the model group. Conclusion Electroacupuncture can improve the depressive state of WKY rats, which may be related to the upregulation of mTOR and downregulation of GSK-3β in the prefrontal cortex.