Objective The objective of this study is to explore the reform significance and effect of integrating regional characteristic cultural courses into cross-cultural nursing education. Methods Reform the teaching method of the "National Medicine Nursing" course, and organically integrate the cross-cultural nursing theory and minority cross-cultural nursing education into the teaching content. A total of 263 undergraduate nursing students in the 2020 class of a Chinese medicine university were selected as the research objects, and the nursing students were divided into an observation group (elective, 125 students) and a control group (no elective, 138 students) according to whether they took the "National Medicine Nursing" course. The differences in the basic conditions of the two groups were compared before teaching, and other educational interventions were monitored during the study period. After teaching, the cross-cultural nursing ability of nursing students in the two groups was measured and compared by using the nursing staff cultural nursing ability questionnaire. The cross-cultural nursing basic knowledge level of nursing students in the two groups was measured and compared, and the course teaching satisfaction was investigated. Results There was no significant difference between the two groups in terms of age, gender, ethnicity, national cultural foundation, and cross-cultural nursing ability level before teaching (P>0.05). The scores of the experimental group (227.37 ± 27.77) and the control group (219.44 ± 27.39) in the post-teaching cultural nursing ability questionnaire were statistically significant(P<0.05). The scores of the level of basic knowledge of the minority cross-cultural nursing for the experimental group and the control group were 59.69 ± 12.45 and 54.24 ± 12.12， respectively, while the difference was statistically significant(P<0.05). The students gave a good evaluation of the course teaching. Conclusion Facing the current situation of cultural integration, cross-cultural nursing ability should be regarded as one of the core competencies of nursing workers. The integration of cross-cultural nursing education into regional characteristic cultural courses conforms to the development of the times and professional needs. The content of cross-cultural nursing education should be more targeted and operable, so as to effectively enhance students?蒺 awareness, knowledge, ability and personal quality of cross-cultural nursing. However, the cross-cultural nursing ability of nursing students needs to be further improved. The law of ability development should be emphasized and the education of cross-cultural nursing should be further strengthened.