With the rapid development of science and technology, gait analysis can reflect the degree of knee osteoarthritis patients and evaluate their walking ability by detecting biomechanical parameters, and provide specific data as a basis for the treatment and rehabilitation training of patients. This paper briefly describes the gait biomechanical basis and pathological gait mechanism of knee osteoarthritis, and briefly analyzes the gait characteristics of knee osteoarthritis patients, so as to provide a basis for the diagnosis and evaluation of knee osteoarthritis.