Objective To investigate the role of LncRNA phenotype in the pathogenesis of acute gouty arthritis (AGA). Methods According to the inclusion criteria, 5 patients with AGA were enrolled in this study and treated with Qingre Tongluo Formula granule for 5 days. Five healthy subjects with non-gouty arthritis(HC) were selected without any treatment. LncRNAs were collected from peripheral blood mononuclear cells(PBMCS) of AGA patients, NAGA patients and HC patients, respectively. Arraystar LncRNAs Microarray technology was used to observe the phenotypic differences of LncRNAs among the three groups. Results Compared with HC group, a total of 2 364 LncRNAs showed phenotypic differences in AGA group, of which 1 174 were up-regulated and 1 190 were down-regulated(Fold Change＞2, P＜0.05). Compared with HC group, a total of 802 LncRNAs were phenotypically different in NAGA group, including 147 up-regulated LncRNAs and 655 down-regulated LncRNAs (Fold Change＞2, P＜0.05). Compared with AGA group, 171 LncRNAs showed phenotypic differences in NAGA group, including 30 up-regulated LncRNAs and 141 down-regulated LncRNAs(Fold Change＞2, P＜0.05). A total of 79 lncrnas were associated among AGA, NAGA and HC groups, among which 68 LncRNAs were up-regulated in AGA group and down-regulated in NAGA group, and 11 LncRNAs were down-regulated in AGA group and up-regulated in NAGA group. Conclusion The phenotypic differences of LncRNAs may be related to the acute onset of gout.