Objective To locate the relevant tenderness sensitization acupoints/locations by detecting the tenderness threshold of acupoint/locations located in cervical and dorsal segments of the governor vein and bladder meridian of patients with cervical spondylosis and comparing with themselves control point and healthy subjects. Methods A digital display mechanical pressure pain meter was used to determine the tenderness threshold of 35 patients with cervical spondylosis and 30 healthy subjects at the acupoints/locations along the cervical and dorsal routes of the governor vein and bladder. The acupoints of tenderness sensitization were determined by comparing with their own non-sensitized parts and the corresponding parts in the healthy group. Results The location of the acupoints/locations associated with cervical spondylopathy were C1 ~ C5 subspinous process of the second lateral line of the bladder, C3 ~ C5 subspinous process of the first lateral line, and C3 subspinous process of the cervical segment of the governor vein, respectively; The optimal and suboptimal sensitizing acupoints/locations were subspinous process of C3 and C4 in the first and second lateral lines of the cervical segment of the bladder and subspinous process of C3 in the cervical segment of the governor vein, respectively. No tenderness sensitive acupoints/locations were detected in the governor vein and bladder meridian on the back. Conclusion In patients with cervical spondylotic disease, there are tenderness sensitive acupoints/locations related to the governor vein and bladder meridian in the neck. Its location can provide reference for precise acupoint selection in clinical treatment of cervical spondylosis.