Objective To study the chemical constituents of the ethyl acetate extract of Aralia chinensis L. var. nuda. Nakai. Methods The ethyl acetate extract of A. chinensis L. var. nuda. Nakai was isolated and purified by silica gel, RP-18, Sephadex LH-20, HPLC and other chromatographic methods, and then the structures were identified by MS, NMR and other spectroscopic methods. Results Fourteen compounds were isolated, and their structures were identified as pentadecanol (1), tetratriacontanole (2), pentadecanoic acid (3), heptadecanoic acid (4), arachidic acid (5), balansenate Ⅰ(6), methyl azelate(7), methyl linolenate(8), octacosanol(9), palmitic acid(10), sitosterol(11), α-spinasterol-3-O-β-D-glucopyranoside (12), 3-O-β-D-glucopyranosyl-stigmasterol (13), β-sitosterol (14). Conclusion Compounds 1~8, 1~14 are separated from Aralia chinensis L. var. nuda. Nakai for the first time. Among them, compounds 1~8, 11~13 were first isolated from the genus Aralia, and compounds 1~7, 12~13 were first separated from the family Araliaceae.