Abstract:Abstract: Objective To study the flavonoids from Daphne pedunculata H. F. Zhou ex C. Y. Chang. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-HPLC, and their structures were elucidated by NMR and mass spectrometry. Results 21 flavonoids were isolated from the 95 % ethanol extract of Daphne pedunculata, 12 of which were biflavones. They were identified as isochamaejasmin (1), neocohamaejasmin A (2), neocohamaejasmin B (3), wikstrol B (4), daphnodirin D2 (5), wikstrol A (6), genikwanol A (7), daphnodirin B (8), wikstaiwanone B (9), wikstaiwanone A (10), daphnodirin G (11), apigenin (12), luteolin (13), genkwanin (14), astragalin (15), apigenin-7-O-glucoside (16), kaempferol (17), luteoloside-7-glucoside luteolin (18), acacetin (19), daphnodin F (20), 5-methoxy-apigenin-7-β-D-glucoside (21). Conclusion Among the 21 flavonoid compounds isolated from the 95% ethanol extract of Daphne pedunculata, except compounds 12, 13 and 14, the others were isolated from Daphne pedunculata for the first time. The research results have enriched the chemical diversity of flavonoids in the plant of Daphne pedunculata, and provided an important foundation and scientific basis for its in-depth research and utilization.