Objective To determine 11 components in Sanhuang Capsule by HPLC as a quality control method. Methods The chromatographic column was selected Agilent Extend-C18 column (250 mm × 4.6 mm, 5 μm), methanol-phosphoric acid aqueous solution with pH 2.5 was used as the mobile phase for gradient elution at a rate of 1 mL·min-1, the detection wavelength was 280 nm, the column temperature was 30 ℃ and the injection volume was 10 μL. Results The average recoveries were 99.15%-116.70%. The average content of berberine hydrochloride, baicalin, quercetin, wogonoside, baicalein, wogonin, aloe-emodin, rhein, emodin, chrysophanol, and physcion were(10.14 ± 0.93)、(12.88 ± 1.12)、(1.71 ± 0.17)、(2.78 ± 0.22)、(0.21 ± 0.01)、(0.20 ± 0.06)、(0.21 ± 0.04)、(0.53 ± 0.04)、(0.35 ± 0.09)、(0.64 ± 0.05)、(0.23 ± 0.04 ) mg/grain. Conclusion The method is simple, stable and reproducible, and can be used as the quality control standard of Sanhuang Capsule.