Objective To study the effect of musk and frankincense on the expression levels of protein kinase-C (PKC) and vascular endothelial growth factor (VEGF) in the treatment of chronic nonbacterial prostatitis. Methods 4 SD rats were selected for the preparation of purified prostatic protein solution. A chronic nonbacterial prostatitis model was established in 48 male NoD mice in 6-week-old except blank control group, and then randomly divided into 7 groups: model group, Phobolate (PMA), high-dose musk-frankincense group, musk-frankincense group, low-dose musk-frankincense group, musk-frankincense group, musk-frankincense group, and frankincense group. The prostatic tissue was isolated and the pathological changes of prostatic tissue were observed by HE staining. Western Blot (WB) was used to detect the expression of PKC and VEGF in prostate tissue. The mechanism of musk + frankincense in the treatment of chronic nonbacterial prostatitis was investigated by pathological examination, PKA and VEGF levels. Results The degree of inflammatory invasion among all groups was compared from high to low as model group > PMA group > Musk and Frankincense group > low-dose musk-Frankincense group > high-dose musk-Frankincense group > blank group. Compared with model group (P<0.05), the PKC expression levels in PMA group < blank group < model group < Musk group, frankincense group < low-dose musk-frankincense group < musk-frankincense group < high-dose musk-frankincense group(P<0.05); The expression levels of VEGF were successively from low to high in blank group < model group < Musk group, frankincense group < low-dose musk-Frankincense group < musk-Frankincense group < high-dose musk-frankincense group(P<0.05) < PMA group(P<0.05). Conclusion Musk + frankincense can up-regulate the expression level of PKC in the pathological state of non-bacterial chronic inflammation. Although it has an effect on the expression level of downstream VEGF, it also plays a regulatory role in treatment, and the relevant mechanism of action needs to be further studied.