Objective To study the effect of interventional Tuina at different time points in the acute period and continuous Tuina after blunt contusion of skeletal muscle in rabbits. Methods Forty rabbits were randomly divided into 5 groups: 30 min postinjury manipulation group, 2 h postinjury manipulation group, ice compress control group, injury observation groupand normal control group. The right gastrocnemius muscle blunt trauma model was created in all groups except the normal control group. The samples were collected and observed on the 7th and 14th day after injury. Results The swelling of the 30 min group was less than that of the 2 h group on the 1st and 2nd day after injury(P<0.05). There was no difference in wet weight ratio of gastrocnemius muscle between 30 min group and 2 h group on day 7 and day 14(P>0.05). After 7 days of injury, the contents of PGE2 and 5-HT in 30 min group were lower than those in 2 h group (P<0.05). After 14 days of injury, the content of IL-6 in 30 min group was lower than that in 2 h group(P<0.05). 7 days after injury, the expression of PAX7 in the 30 min group was lower than that in the 2 h group (P<0.05). After 14 days of injury, the expression of MyoD in 30 min group and 2 h group tended to be consistent, and the expression of PAX7 in 30 min group was lower than that in 2 h group(P<0.05). Conclusion In acute period of blunt trauma of skeletal muscle in rabbits, the quality of 30 min Tuina is better than 2 h Tuina.