白果仁35%乙醇水洗脱部位多糖及多肽的含量测定
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1.云南中医药大学中药学院;2.淄博市市级机关医院;3.中国中医科学院中药研究所道地药材品质保障与资源持续利用全国重点实验室

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基金项目:

]国家自然科学(82374022),中国中医科学院科技创新工程项目资助(CI2023E002,CI2021A04409)中央级公益性科研院所基本科研业务费专项资金资助(No. ZZ13-YQ-061,ZXKT22012,ZXKT22039)


Determination of polysaccharide and polypeptide content in 35 % ethanol water elution fraction of ginkgo kernels
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Affiliation:

1.Traditional Chinese Medicine College of Yunnan University of Chinese Medicine;2.Zibo Municipal Organ Hospital;3.National Key Laboratory of Quality Assurance and Sustainable Utilization of Genuine Medicinal Materials,Institute of Traditional Chinese Medicine,Chinese Academy of Chinese Medical Sciences

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    摘要:

    目的:对白果仁止咳有效部位35%乙醇水洗脱部位中多糖及多肽的含量进行测定,为白果仁的综合开发和质量控制提供科学依据。方法:采用苯酚-硫酸法、双缩脲法,建立标准曲线,并进行精密度、稳定性、重复性、加样回收率的考察。结果:白果仁35%乙醇水洗脱部位多糖的含量占13.99%,多肽的含量占31.41%,二者均在浓度范围内有良好的线性关系,且加样回收率分别为97.96%、97.94%。结论:本研究建立的白果仁35%乙醇水洗脱部位多糖和多肽含量测定方法简单可行,稳定性和重现性好。

    Abstract:

    Objective : To determine the content of polysaccharides and polypeptides in the 35% ethanol water elution part of the effective part of ginkgo kernels, and to provide scientific basis for the comprehensive development and quality control of ginkgo kernels. Methods : The standard curve was established by phenol-sulfuric acid method and biuret method, and the precision, stability, repeatability and recovery rate were investigated. Results : The content of polysaccharide in the 35% ethanol water elution part of ginkgo kernels accounted for 13.99 %, and the content of polypeptide accounted for 31.41%. Both of them had a good linear relationship in the concentration range, and the recovery rates were 97.96% and 97.94%. Conclusion : The established method for the determination of polysaccharides and polypeptides in the 35% ethanol water elution part of ginkgo kernels is simple and feasible, with good stability and reproducibility.

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  • 收稿日期:2024-02-26
  • 最后修改日期:2024-05-06
  • 录用日期:2024-05-06
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