Objective To explore the effect of Yijing Huoxue Fang with Vitamin E on abnormal sperm DNA fragmentation index with kidney deficiency and blood stasis men. Methods Follow the inclusion criteria investigation has made, 60 patients diagnosed as abnormal sperm DNA fragmentation index with kidney deficiency and blood stasis in Chinese medicine syndrome differentiation, divided into treatment group and control group according to the treatment needs of the patients, 30 cases were included in the two groups respectively. Treatment group prescription to Yijing Huoxue Fang and Vitamin E,combine with lifestyle intervention therapy; control group adopt lifestyle intervention treatment, lasting for 3 months.Observe sperm DNA fragmentation index、 sperm concentration、 sperm motility(PR, PR+NP)、 normal sperm morphology rate before and after treatment,and the clinical efficacy Evaluated. Results Use t-test compare sperm DNA fragmentation index before and after treatment, there was significant decreased after treatment in both treatment group and control group(P＜0.05); and the treatment group was lower than the control group after treatment, there was significant statistical difference(P＜0.05). in parameters of semen, no significant difference were observed by sperm concentration、 total sperm count、 progressive motility, total motility and normal sperm morphology rate in control group between before and after treatment(P＞0.05); in treatment group, total sperm count after treatment was increased than before,but no significant difference(P＞0.05), in sperm concentration, progressive motility, total motility and normal sperm morphology rate, they were significant improvement(P＜0.05); after treatment, the progressive motility、 total motility and normal morphology of the treatment group were higher than those of the control group, and the difference were statistically significant(P＜0.05). Conclusion Lifestyle interventions and the combination of Yijinghuoxue Fang and vitamin E can both reduce the sperm DNA fragmentation index,and Yijinghuoxue fang with Vitamin E more effectively, and has significant therapeutic effects on the progressive motility、 total motility and normal morphology.