Objective Exploring the effects of acupoint catgut embedding on hypothalamic pituitary adrenal (HPA) axis related hormones and inflammatory factors in the serum of IBS-D rats. Methods According to the random number method,18SD rats were randomly divided into anormal group,amodel group,and a catgut embedding group,with 6rats in each group.The IBS-D rat model was established using chronic stress (restraint+ excessive fatigue + dietary disorders)+senna decoction gavage method.The catgut embedding group was treated with catgut embedding intervention at the acuintsfnsh,P,TichgTZnsi,tinrigertotal of three times. Ater the intervention,sampleswere collected from ratsfor fecal trait scoring and visceral painthreshold testing; enzyme linked immunosorbent assay (ELISA) was used to detect the levels of HPA axis related hormones corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosrterone (Cort), and inflammatory factors interleukin (IL)-1β and IL-10 in serum. Results Afer the intervention, compared with the normal group, the fecal trai score of the IBS-D model group rats increased(P