Objective To investigate the hypoglycemic effect of A. muricata root extract on spontaneous type 2 diabetes db/db mice. Methods Spontaneous type 2 diabetes db/db mice were used as a model, and db/db mice were randomly divided into: model group, administration group(A. muricata root extract, 200 mg·kg-1), positive drug group (metformin, 200 mg·kg-1). Another blank group(db/+ mice) was set up. After 8 weeks of continuous intragastric administration, fasting blood glucose, and measuring body weight, food intake and water intake weekly, and glycated hemoglobin (HbA1c) levels were measured. The levels of serum triglyceride(TG), low density lipoprotein(LDL) and total cholesterol(TC) were detected after administration. Calculating HOMA-IR index and HOMA-β index. The histopathological changes of liver, kidney and pancreas in db/db mice were observed by HE staining. Results With 200 mg·kg-1 A. muricata root extract was shown to increase body weight, reduce food and water intake, and decrease fasting blood glucose, HbA1c, triglyceride (TG) levels, and HOMA-β index in db/db mice; The pathological conditions of liver, kidney and pancreas in mice were significantly improved. Conclusion The A. muricata root extract has a good therapeutic effect on type 2 diabetes, this study provides a scientific basis for the application and development of A. muricata root as a treatment for type 2 diabetes.