Strain the F. proliferatum RY-20 was cultivated with the addition of a epigenetic modifier, suberoylanilide hydroxamic acid(SAHA) to study the secondary metabolites. The monomer compounds were isolated and purified from the fermentation extract by silica gel column chromatography and dextran gel(Sephadex LH-20) column chromatography. The structures of compounds were identified by spectral technique and literature data. Finally, the antimicrobial activities of these compounds were tested by Disk Diffusion Assay. The results were as follows: (1) Ten compounds were isolated from fermentation cultures of SAHA-supplemented F. proliferatum RY-20 culture and identified as 1-monolinolein (1), (4E,8E)-N-D-2′-hydroxyoctadecanoyl-1-O-β-D-glycopyranosyl-9-methyl-4,8-sphinga dienine(2), adenosine(3), 3β,5α-dihydroxy-ergosta-7,22-dien-6-one (4), 3β-hydroxy-5,9-epoxy-(22E,24R)-ergosta-7,22-dien-6-one (5), 5α,6α,8α,9α-diepoxy-(22E,24R)-ergost-22-ene-3β,7α-diol(6), (22E,24R)-ergosta-7,22-dien-3β,5α,6β,9α-tetraol(7), 19-norergosta-5,7,9,22-tetraene-3β-ol(8), bis(2-ethylhexyl) phthalate(9), D-threitol(10). In comparison to the metabolites from monoculture, compounds 1 and 9 were induced by adding SAHA to F. proliferatum RY-20, and isolated for the first time from this strain. (2) Compounds 1~10 exhibited weak antimicrobial activities at the dose of 50 μg.