Objective To explore the mechanism of "softening tendons and warming Tong" manipulation to intervene chronic skeletal muscle injury in rabbits. Methods 40 healthy adult rabbits were selected, 30 rabbits were set as experimental group, control group and model group, and the remaining 10 rabbits were set as normal group. The experimental group used "soft tendons warming Tong" manipulation intervention, the control group used dexamethasone drug treatment, co-treatment 10 times, the model group and the normal group were fed normally. After treatment, samples were collected for detection, and TNF-α and IL-6 were detected by ELISA, and the protein expression of TGF-β1 and CTGF were detected by Western blot. Results The contents of TNF-α and IL-6 in skeletal muscle were significantly decreased.The protein expressions of CTGF and TGF-β1 were significantly decreased. Conclusion Through the study, "softening tendons and warming Tong" manipulation can reduce the content of local inflammatory factors (TNF-α, IL-6) in skeletal muscle injury as well as reduce the content of inflammatory factors TGF-β1, CTGF protein expression, to inhibit inflammatory adhesion and inflammatory fibrous tissue hyperplasia, promote skeletal muscle chronic damage repair of injury.